Faekosianin e e Botala jwa legodimo
Tlhaloso
Phycocyanin (C-PC) ke poroteine e e nwelelang mo metsing, e e botala jwa legodimo e e tswang thata mo dibakateria tsa cyanobaketeria tse di jaaka Spirulina platensis le Arthrospira maxima. E dira jaaka mmala o o kopang lesedi mo photosynthesis fa e ntse e bontsha dithoto tse di maatla tsa antioxidant, go lwantsha thurugo, le go fetola masole a mmele.
Dipharologantsho tsa Dikhemikhale le tsa Popego:
Bokete jwa Molekhule: ~240 kDa (sebopego sa heksameriki)
Setlhoa sa Monyelo: 620 nm (mmala o o botala jo bo galotseng)
Go phatsima: E ntsha ka 650 nm (thobo ya quantum ~0.7)
Dipharologantsho tsa Sepekterosekopo
| Pharametara | Tlhwatlhwa | Botlhokwa |
| Segokanyipalo sa go nyelela (ε620) | 1.54×106 M-1cm-1 | Maemo a dipalopalo |
| Botshelo jwa go phatsima | 2.8 ns | Botsitso jwa patlisiso |
| Thobo ya bontsi | 0,68±0,03 | Ditiriso tsa go tsaya ditshwantsho |
| Go tlhomama ga lesedi | t1⁄2 >8h (lesedi le le bonalang) | Go akanyetsa go tlhama |
Go tswakana:
Metsi: A tswakana thata (>50 mg/mL)
Ethanole: E tlhotlhorega go sekae (5-10 mg/mL)
Ga e nwelelele mo ditlhaolosing tsa dibodi (sekao, tlelorofomo, heksane)
Go ntsha le go itshekisa:
Go Ntshiwa ga Metsi a a Tsididi: Go boloka bioactivity (thobo: 10-15% go tswa mo biomass e e omileng).
CO2 e e botlhokwa thata: E tokafatsa bophepa (>95%) ka 17 MPa, 45°C.
Chromatokerafi: Go monyelwa ga bolao go go atolositsweng (EBA) ga C-PC e e phepa thata
Mekgwa ya Tshekatsheko
Bophepa: Sepekterofotometeri sa UV-Vis
Boitshupo: TSEBE ya Selegae + go tshasiwa ga zinki
Dikgotleletsi: LC-MS/MS (botlhole jwa dimela Tiro: Teko ya bokgoni jwa go monya okosejene
Dipharametara tsa Boleng
| Seemo | Bophepa (A20/A220) | Dikopo |
| Dijo | >0.7 | Mmala wa tlhago |
| Ditlolo | >2.0 | Ditlhamo tsa mo sefatlhegong |
| Dikotla | >3.0 | Ditlaleletsi tsa dijo |
| Patlisiso | >4.0 | Diteko tsa baeyokhemikhale |
Mesola
✔ Sethibela-dikotsi se se Maatla – Se fokotsa di-radical tse di sa duelelweng
✔ E lwantsha thurugo – E fokotsa thurugo e e sa foleng
✔ Tshegetso ya Masole a Mmele – E tokafatsa tiro ya disele tsa madi
✔ Tshireletso ya methapo – E tshegetsa boitekanelo jwa boboko
✔ Mmala wa Tlhago – Sefepi sa dijo se se pududu se se amogetsweng ke FDA
Dikopo
Dikotla: Dikhasepole, dipoere (100-500 mg / letsatsi)
Dijo tse di dirang: Aesekerimi, dino (mmala wa botala jwa loapi jwa tlholego)
Ditlolo: Diserum tse di lwantshang botsofe, ditlolo tse di phatsimang
Patlisiso ya Kalafi ya Ditshedi: Dipatlisiso tsa fluorescent, go tlisa melemo
Go Dira Dikumo












